Sensitive 13C-13C correlation spectra of amyloid fibrils at very high spinning frequencies and magnetic fields

Markus Weingarth; Yuichi Masuda; K. Takegoshi; Geoffrey Bodenhausen; Piotr Tekely

doi:10.1007/s10858-011-9501-9

Sensitive ¹³C-¹³C correlation spectra of amyloid fibrils at very high spinning frequencies and magnetic fields

Markus Weingarth, Yuichi Masuda, K. Takegoshi, Geoffrey Bodenhausen, Piotr Tekely

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Sensitive 2D solid-state ¹³C-¹³C correlation spectra of amyloid β fibrils have been recorded at very fast spinning frequencies and very high magnetic fields. It is demonstrated that PARIS-xy recoupling using moderate rf amplitudes can provide structural information by promoting efficient magnetization transfer even under such challenging experimental conditions. Furthermore, it has been shown both experimentally and by numerical simulations that the method is not very sensitive to dipolar truncation effects and can reveal direct transfer across distances of about 3.5-4Å .

Original language	English
Pages (from-to)	129-136
Number of pages	8
Journal	Journal of biomolecular NMR
Volume	50
Issue number	2
DOIs	https://doi.org/10.1007/s10858-011-9501-9
Publication status	Published - 2011 Jun
Externally published	Yes

Keywords

Amyloid β
Dipolar truncation
Fibrils
PARIS-xy dipolar recoupling
Solid-state NMR
Ultra-high magnetic field

ASJC Scopus subject areas

Biochemistry
Spectroscopy

Access to Document

10.1007/s10858-011-9501-9

Cite this

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title = "Sensitive 13C-13C correlation spectra of amyloid fibrils at very high spinning frequencies and magnetic fields",

abstract = "Sensitive 2D solid-state 13C-13C correlation spectra of amyloid β fibrils have been recorded at very fast spinning frequencies and very high magnetic fields. It is demonstrated that PARIS-xy recoupling using moderate rf amplitudes can provide structural information by promoting efficient magnetization transfer even under such challenging experimental conditions. Furthermore, it has been shown both experimentally and by numerical simulations that the method is not very sensitive to dipolar truncation effects and can reveal direct transfer across distances of about 3.5-4{\AA} .",

keywords = "Amyloid β, Dipolar truncation, Fibrils, PARIS-xy dipolar recoupling, Solid-state NMR, Ultra-high magnetic field",

author = "Markus Weingarth and Yuichi Masuda and K. Takegoshi and Geoffrey Bodenhausen and Piotr Tekely",

note = "Funding Information: Acknowledgments Financial support from the Agence Nationale de la Recherche (ANR-09-BLAN-0111-01) and from the F{\'e}d{\'e}ration de Recherche (FR 3050) Tr{\`e}s Grands Equipements de R{\'e}sonance Magn{\'e}tique Nucl{\'e}aire {\`a} Tr{\`e}s Hauts Champs (TGE RMN THC) of the CNRS is gratefully acknowledged. We thank Prof. Kazuhiro Irie at the Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, for the use of a peptide synthesizer. We are grateful to Dr. Ken-ichi Akagi, Ms. Youko Monobe, and",

year = "2011",

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doi = "10.1007/s10858-011-9501-9",

language = "English",

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AU - Weingarth, Markus

AU - Masuda, Yuichi

AU - Takegoshi, K.

AU - Bodenhausen, Geoffrey

AU - Tekely, Piotr

N1 - Funding Information: Acknowledgments Financial support from the Agence Nationale de la Recherche (ANR-09-BLAN-0111-01) and from the Fédération de Recherche (FR 3050) Très Grands Equipements de Résonance Magnétique Nucléaire à Très Hauts Champs (TGE RMN THC) of the CNRS is gratefully acknowledged. We thank Prof. Kazuhiro Irie at the Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, for the use of a peptide synthesizer. We are grateful to Dr. Ken-ichi Akagi, Ms. Youko Monobe, and

PY - 2011/6

Y1 - 2011/6

N2 - Sensitive 2D solid-state 13C-13C correlation spectra of amyloid β fibrils have been recorded at very fast spinning frequencies and very high magnetic fields. It is demonstrated that PARIS-xy recoupling using moderate rf amplitudes can provide structural information by promoting efficient magnetization transfer even under such challenging experimental conditions. Furthermore, it has been shown both experimentally and by numerical simulations that the method is not very sensitive to dipolar truncation effects and can reveal direct transfer across distances of about 3.5-4Å .

AB - Sensitive 2D solid-state 13C-13C correlation spectra of amyloid β fibrils have been recorded at very fast spinning frequencies and very high magnetic fields. It is demonstrated that PARIS-xy recoupling using moderate rf amplitudes can provide structural information by promoting efficient magnetization transfer even under such challenging experimental conditions. Furthermore, it has been shown both experimentally and by numerical simulations that the method is not very sensitive to dipolar truncation effects and can reveal direct transfer across distances of about 3.5-4Å .

KW - Amyloid β

KW - Dipolar truncation

KW - Fibrils

KW - PARIS-xy dipolar recoupling

KW - Solid-state NMR

KW - Ultra-high magnetic field

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