Sensitive ferrocene reagents for derivatization of thiol compounds in high-performance liquid chromatography with dual-electrode coulometric dectection

Kazutake Shimada, Tomoyuki Oe, Toshio Nambara

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Three N-substituted maleimides possessing ferrocene as an electrophore were prepared and evaluated for pre-column derivatization of thiol compounds in high-performance liquid chromatography with dual-electrode coulometric detection. The utility of these reagents was investigated by employing N-acetyl-L-cysteine as a model compound. Among the three, N-(ferrocenyl) maleimide was the most favourable reagent with respect to reactivity, stability and electrochemical properties. The dual-electrode coulometric detection of the adduct showed high selectivity and sensitivity with a detection liming of 0.06 pmol. The proposed method is applicable to the determination of glutathione in biological specimens.

Original languageEnglish
Pages (from-to)17-25
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume419
Issue numberC
DOIs
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Chemistry(all)

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