Three N-substituted maleimides possessing ferrocene as an electrophore were prepared and evaluated for pre-column derivatization of thiol compounds in high-performance liquid chromatography with dual-electrode coulometric detection. The utility of these reagents was investigated by employing N-acetyl-L-cysteine as a model compound. Among the three, N-(ferrocenyl) maleimide was the most favourable reagent with respect to reactivity, stability and electrochemical properties. The dual-electrode coulometric detection of the adduct showed high selectivity and sensitivity with a detection liming of 0.06 pmol. The proposed method is applicable to the determination of glutathione in biological specimens.
|Number of pages||9|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Publication status||Published - 1987|
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