TY - JOUR
T1 - Sensitive ferrocene reagents for derivatization of amines for high-performance liquid chromatography with electrochemical detection
AU - Shimada, Kazutake
AU - Oe, Tomoyuki
AU - Tanaka, Makoto
AU - Nambara, Toshio
PY - 1989
Y1 - 1989
N2 - Six reagents possessing ferrocene as an electrophore were prepared and evaluated for pre-column derivatization of amino compounds for their determination by high-performance liquid chromatography with electrochemical detection. The utility of these reagents was investigated employing phenethylamine as a model compound. Among these six, N-succinimidyl 3-ferrocenylpropionate was the best with respect to reactivity, stability and electrochemical properties. The developed method was applied to the determination of putrescine formed from ornithine by ornithine decarboxylase.
AB - Six reagents possessing ferrocene as an electrophore were prepared and evaluated for pre-column derivatization of amino compounds for their determination by high-performance liquid chromatography with electrochemical detection. The utility of these reagents was investigated employing phenethylamine as a model compound. Among these six, N-succinimidyl 3-ferrocenylpropionate was the best with respect to reactivity, stability and electrochemical properties. The developed method was applied to the determination of putrescine formed from ornithine by ornithine decarboxylase.
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U2 - 10.1016/S0378-4347(00)83034-0
DO - 10.1016/S0378-4347(00)83034-0
M3 - Article
C2 - 2722995
AN - SCOPUS:0024311925
VL - 487
SP - 247
EP - 255
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - C
ER -