TY - JOUR
T1 - Sensitive detection of intracellular environment of normal and cancer cells by autofluorescence lifetime imaging
AU - Awasthi, Kamlesh
AU - Moriya, Daiki
AU - Nakabayashi, Takakazu
AU - Li, Liming
AU - Ohta, Nobuhiro
N1 - Funding Information:
N. O. thanks Professor Yuan-Pern Lee (National Chiao Tung University) for generous support. National Chiao Tung University , Ministry of Science and Technology and the MOE-ATU program of Taiwan provided support to K. A and N. O. This work was also supported by Research Institute for Electronic Science, Hokkaido University, Japan .
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Intracellular fluorescence lifetime images of the endogenous fluorophores of nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD), which are well known as autofluorescence chromophores, were obtained from rat normal fibroblast cells (WFB) and H-ras oncogene-transfected cancer cells among WFB (W31). The average lifetime of the NADH and FAD autofluorescence was shorter in cancer cells than in normal cells, indicating that the difference in metabolism between healthy and cancer cells alters the conditions for coenzymes such as NADH and FAD and that the autofluorescence lifetime measurement of NADH and FAD is applicable for the noninvasive diagnosis of cancer cells. The pico- and nano-second time-resolved fluorescence spectra of NADH obtained with different time windows were similar in normal and cancer cells, indicating that every fluorescence decay component gives the same spectrum in both cell types. These results as well as the fluorescence lifetime images of exogenous fluorophores stained with sodium pheophorbide a in normal and cancer cells suggest that the difference in the fluorescence lifetime between normal and cancer cells cannot be attributed to a difference in the intracellular pH or refractive index but to the difference in the bound condition between proteins and NADH or FAD under the different intracellular environments of normal and cancer cells.
AB - Intracellular fluorescence lifetime images of the endogenous fluorophores of nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD), which are well known as autofluorescence chromophores, were obtained from rat normal fibroblast cells (WFB) and H-ras oncogene-transfected cancer cells among WFB (W31). The average lifetime of the NADH and FAD autofluorescence was shorter in cancer cells than in normal cells, indicating that the difference in metabolism between healthy and cancer cells alters the conditions for coenzymes such as NADH and FAD and that the autofluorescence lifetime measurement of NADH and FAD is applicable for the noninvasive diagnosis of cancer cells. The pico- and nano-second time-resolved fluorescence spectra of NADH obtained with different time windows were similar in normal and cancer cells, indicating that every fluorescence decay component gives the same spectrum in both cell types. These results as well as the fluorescence lifetime images of exogenous fluorophores stained with sodium pheophorbide a in normal and cancer cells suggest that the difference in the fluorescence lifetime between normal and cancer cells cannot be attributed to a difference in the intracellular pH or refractive index but to the difference in the bound condition between proteins and NADH or FAD under the different intracellular environments of normal and cancer cells.
KW - Autofluorescence
KW - Cancer cell
KW - FAD
KW - FLIM
KW - NADH
KW - Normal cell
UR - http://www.scopus.com/inward/record.url?scp=84995468207&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84995468207&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2016.10.023
DO - 10.1016/j.jphotobiol.2016.10.023
M3 - Article
C2 - 27842280
AN - SCOPUS:84995468207
VL - 165
SP - 256
EP - 265
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
ER -