Semaphorin controls epidermal morphogenesis by stimulating mRNA translation via eIF2α in Caenorhabditis elegans

Akira Nukazuka, Hajime Fujisawa, Toshifumi Inada, Yoichi Oda, Shin Takagi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Conserved semaphorin-plexin signaling systems govern various aspects of animal development, including axonal guidance in vertebrates and epidermal morphogenesis in Caenorhabditis elegans. Here we provide in vivo evidence that stimulation of mRNA translation via eukaryotic initiation factor 2α (eIF2α) is an essential downstream event of semaphorin signaling in C. elegans. In semaphorin/plexin mutants, a marked elevation in the phosphorylation of eIF2α is observed, which causes translation repression and is causally related to the morphological epidermal phenotype in the mutants. Conversely, removal of constraints on translation by genetically reducing the eIF2α phosphorylation largely bypasses requirement for the semaphorin signal in epidermal morphogenesis. We also identify an actin-depolymerizing factor/cofilin, whose expression in the mutants is predominantly repressed, as a major translational target of semaphorin signaling. Thus, our results reveal a physiological significance for translation of mRNAs for cytoskeletal regulators, linking environmental cues to cytoskeletal rearrangement during cellular morphogenesis in vivo.

Original languageEnglish
Pages (from-to)1025-1036
Number of pages12
JournalGenes and Development
Volume22
Issue number8
DOIs
Publication statusPublished - 2008 Apr 15
Externally publishedYes

Keywords

  • C. elegans
  • Cofilin
  • Epidermal morphogenesis
  • Semaphorin
  • eIF2
  • mRNA translation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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