TY - JOUR
T1 - Selfish prion of Rnq1 mutant in yeast
AU - Kurahashi, Hiroshi
AU - Shibata, Shoichiro
AU - Ishiwata, Masao
AU - Nakamura, Yoshikazu
PY - 2009/5/8
Y1 - 2009/5/8
N2 - [PIN+]is a prion form of Rnq1 in Saccharomyces cerevisiae and is necessary for the de novo induction of a second prion, [PSI+]. We previously isolated a truncated form of Rnq1, named Rnq1Δ100, as a [PSI+]-eliminating factor in S. cerevisiae. Rnq1Δ100 deletes the N-terminal non-prion domain of Rnq1, and eliminates [PSI+]in [PIN+]yeast. Here we found that [PIN+] is transmissible to Rnq1Δ100 in the absence of full-length Rnq1, forming a novel prion variant [RNQ1Δ100+]. [RNQ1Δ100+]has similar [PIN+]properties as it stimulates the de novo induction of [PSI+] and is eliminated by the null hsp104Δ mutation, but not by Hsp104 overproduction. In contrast, [RNQ1Δ100+] inherits the inhibitory activity and hampers the maintenance of [PSI+] though less efficiently than [PIN+]made of Rnq1-Rnq1Δ100 co-aggregates. Interestingly, [RNQ1Δ100+] prion was eliminated by de novo [PSI+] induction. Thus, the [RNQ1Δ100+] prion demonstrates selfish activity to eliminate a heterologous prion in S. cerevisiae, showing the first instance of a selfish prion variant in living organisms.
AB - [PIN+]is a prion form of Rnq1 in Saccharomyces cerevisiae and is necessary for the de novo induction of a second prion, [PSI+]. We previously isolated a truncated form of Rnq1, named Rnq1Δ100, as a [PSI+]-eliminating factor in S. cerevisiae. Rnq1Δ100 deletes the N-terminal non-prion domain of Rnq1, and eliminates [PSI+]in [PIN+]yeast. Here we found that [PIN+] is transmissible to Rnq1Δ100 in the absence of full-length Rnq1, forming a novel prion variant [RNQ1Δ100+]. [RNQ1Δ100+]has similar [PIN+]properties as it stimulates the de novo induction of [PSI+] and is eliminated by the null hsp104Δ mutation, but not by Hsp104 overproduction. In contrast, [RNQ1Δ100+] inherits the inhibitory activity and hampers the maintenance of [PSI+] though less efficiently than [PIN+]made of Rnq1-Rnq1Δ100 co-aggregates. Interestingly, [RNQ1Δ100+] prion was eliminated by de novo [PSI+] induction. Thus, the [RNQ1Δ100+] prion demonstrates selfish activity to eliminate a heterologous prion in S. cerevisiae, showing the first instance of a selfish prion variant in living organisms.
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U2 - 10.1111/j.1365-2443.2009.01297.x
DO - 10.1111/j.1365-2443.2009.01297.x
M3 - Article
C2 - 19371377
AN - SCOPUS:65449168747
VL - 14
SP - 659
EP - 668
JO - Genes to Cells
JF - Genes to Cells
SN - 1356-9597
IS - 5
ER -