Self-formation of bilayer lipid membranes on agarose-coated silicon surfaces studied by simultaneous electrophysiological and surface infrared spectroscopic measurements

Ayumi Hirano-Iwata, Azusa Oshima, Kota Onodera, Kouji Aoto, Tasuku Taira, Ryo Taro Yamaguchi, Yasuo Kimura, Michio Niwano

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Self-formation process of bilayer lipid membranes (BLMs) cushioned on agarose-coated Si surfaces was in situ monitored by simultaneous electrophysiological and infrared absorption spectroscopic (IRAS) measurements using IRAS with the multiple internal reflection geometry. IRAS signals corresponding to self-thinning of lipid solution to form BLMs were demonstrated. It was found that the appearance of IRAS bands due to C=O modes of phosphstidylcholine is related to formation of BLMs with a gigaohm seal. The functionality of the present BLM system was also demonstrated by incorporating gramicidin into the BLMs and recording its channel activities.

Original languageEnglish
Article number243906
JournalApplied Physics Letters
Volume94
Issue number24
DOIs
Publication statusPublished - 2009 Jun 29

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Fingerprint Dive into the research topics of 'Self-formation of bilayer lipid membranes on agarose-coated silicon surfaces studied by simultaneous electrophysiological and surface infrared spectroscopic measurements'. Together they form a unique fingerprint.

  • Cite this