Self-assembling nanoprobes that display off/on 19 F nuclear magnetic resonance signals for protein detection and imaging

Yousuke Takaoka, Takashi Sakamoto, Shinya Tsukiji, Michiko Narazaki, Tetsuya Matsuda, Hidehito Tochio, Masahiro Shirakawa, Itaru Hamachi

Research output: Contribution to journalArticlepeer-review

182 Citations (Scopus)

Abstract

Magnetic resonance imaging (MRI) is one of the most promising techniques for the non-invasive visualization of biomarkers and biologically relevant species, both in vivo and ex vivo. Although 1 H MRI with paramagnetic contrast agents, such as Gd 3 complexes and iron oxide, is widely used, it often suffers from low contrast because of the large background signals caused by the abundant distribution of protons in biological samples. Here we report the use of supramolecular organic nanoparticles to detect specific proteins by 19 F-based MRI in an off/on mode. In NMR spectroscopy these designed probes are silent when aggregated, but in the presence of a target protein they disassemble to produce a sharp signal. This turn-on response allowed us to visualize clearly the protein within live cells by 19 F MRI and construct an in-cell inhibitor assay. This recognition-driven disassembly of nanoprobes for a turn-on 19 F signal is unprecedented and may extend the use of 19 F MRI for specific protein imaging.

Original languageEnglish
Pages (from-to)557-561
Number of pages5
JournalNature Chemistry
Volume1
Issue number7
DOIs
Publication statusPublished - 2009 Oct
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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