Self-assembled nanogel of hydrophobized dendritic dextrin for protein delivery

Yayoi Ozawa, Shin Ichi Sawada, Nobuyuki Morimoto, Kazunari Akiyoshi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Highly branched cyclic dextrin derivatives (CH-CDex) that are partly substituted with cholesterol groups have been synthesized. The CH-CDex forms monodisperse and stable nanogels with a hydrodynamic radii of ≈10 nm by the self-assembly of 4-6 CH-CDex macromolecules in water. The CH-CDex nanogels spontaneously trap 10-16 molecules of fluorescein isothiocyanate-labeled insulin (FITC-Ins). The complex shows high colloidal stability: no dissociation of trapped insulin is observed after at least 1 month in phosphate buffer (0.1 M, pH 8.0). In the presence of bovine serum albumin (BSA, 50 mg · mL-1), which is a model blood system, the FITC-Ins trapped in the nanogels is continuously released (≈20% at 12 h) without burst release. The high-density nanogel structure derived from the highly branched CDex significantly affects the stability of the nanogel-protein complex. A figure is presented.

Original languageEnglish
Pages (from-to)694-701
Number of pages8
JournalMacromolecular Bioscience
Volume9
Issue number7
DOIs
Publication statusPublished - 2009 Aug 19
Externally publishedYes

Keywords

  • Chaperon-like activity
  • Dendrimers
  • Highly branched cyclic dextrin
  • Nanogel
  • Protein delivery
  • Self-assembly

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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