Self-assembled biotinylated disulfide derivative monolayer on gold electrode for immobilizing enzymes

Pingang He; Jiannong Ye; Yuzhi Fang; Junichi Anzai; Tetsou Osa

doi:10.1016/S0039-9140(96)02133-9

Self-assembled biotinylated disulfide derivative monolayer on gold electrode for immobilizing enzymes

Pingang He, Jiannong Ye, Yuzhi Fang, Junichi Anzai, Tetsou Osa

PHA - Molecular Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Based on self-assembled biotinylated disulfide derivative monolayer on gold electrode, the sensors immobilized monolayer or multilayer membranes composed of avidin and biotinlabeled glucose oxidase (B·GOD) or of avidin-B·GOD complex (ABC) and B·COD were prepared. The present technique may be useful for controlling the enzyme content of the sensors in molecular level by repeating the deposition of enzyme layers. The sensors have the characteristics of shorter response time, higher sensitivity. The linear range is from 6.0 x 10^-6-5.0 x 10^-3 M. The sensor can be used for more than 1 month and can be reactivated. The sensor was used to determine glucose in human blood serum, and the results are satisfactory.

Original language	English
Pages (from-to)	885-890
Number of pages	6
Journal	Talanta
Volume	44
Issue number	5
DOIs	https://doi.org/10.1016/S0039-9140(96)02133-9
Publication status	Published - 1997 May

Keywords

Avidin
Biotin
Enzyme sensors
Glucose oxidase
Immobilization
Self-assembled monolayer

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/S0039-9140(96)02133-9

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title = "Self-assembled biotinylated disulfide derivative monolayer on gold electrode for immobilizing enzymes",

abstract = "Based on self-assembled biotinylated disulfide derivative monolayer on gold electrode, the sensors immobilized monolayer or multilayer membranes composed of avidin and biotinlabeled glucose oxidase (B·GOD) or of avidin-B·GOD complex (ABC) and B·COD were prepared. The present technique may be useful for controlling the enzyme content of the sensors in molecular level by repeating the deposition of enzyme layers. The sensors have the characteristics of shorter response time, higher sensitivity. The linear range is from 6.0 x 10-6-5.0 x 10-3 M. The sensor can be used for more than 1 month and can be reactivated. The sensor was used to determine glucose in human blood serum, and the results are satisfactory.",

keywords = "Avidin, Biotin, Enzyme sensors, Glucose oxidase, Immobilization, Self-assembled monolayer",

author = "Pingang He and Jiannong Ye and Yuzhi Fang and Junichi Anzai and Tetsou Osa",

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T1 - Self-assembled biotinylated disulfide derivative monolayer on gold electrode for immobilizing enzymes

AU - He, Pingang

AU - Ye, Jiannong

AU - Fang, Yuzhi

AU - Anzai, Junichi

AU - Osa, Tetsou

PY - 1997/5

Y1 - 1997/5

N2 - Based on self-assembled biotinylated disulfide derivative monolayer on gold electrode, the sensors immobilized monolayer or multilayer membranes composed of avidin and biotinlabeled glucose oxidase (B·GOD) or of avidin-B·GOD complex (ABC) and B·COD were prepared. The present technique may be useful for controlling the enzyme content of the sensors in molecular level by repeating the deposition of enzyme layers. The sensors have the characteristics of shorter response time, higher sensitivity. The linear range is from 6.0 x 10-6-5.0 x 10-3 M. The sensor can be used for more than 1 month and can be reactivated. The sensor was used to determine glucose in human blood serum, and the results are satisfactory.

AB - Based on self-assembled biotinylated disulfide derivative monolayer on gold electrode, the sensors immobilized monolayer or multilayer membranes composed of avidin and biotinlabeled glucose oxidase (B·GOD) or of avidin-B·GOD complex (ABC) and B·COD were prepared. The present technique may be useful for controlling the enzyme content of the sensors in molecular level by repeating the deposition of enzyme layers. The sensors have the characteristics of shorter response time, higher sensitivity. The linear range is from 6.0 x 10-6-5.0 x 10-3 M. The sensor can be used for more than 1 month and can be reactivated. The sensor was used to determine glucose in human blood serum, and the results are satisfactory.

KW - Avidin

KW - Biotin

KW - Enzyme sensors

KW - Glucose oxidase

KW - Immobilization

KW - Self-assembled monolayer

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