TY - JOUR
T1 - Selective upregulation of RB3/stathmin4 by ciliary neurotrophic factor following optic nerve axotomy
AU - Nakazawa, Toru
AU - Morii, Hiroshi
AU - Tamai, Makoto
AU - Mori, Nozomu
N1 - Funding Information:
This work is in part supported by the grant from Grant-in-Aid for Scientific Research from Ministry of Education, Science, and Culture of the Japanese Government (B-2-15390524; MT) and the Research Grants in the Natural Sciences from the Mitsubishi Foundation (NM). The authors thank Lucia Sobrin, M.D. and Haicheng She M.D. Ph.D. (Massachusetts Eye and Ear Infirmary) for editing the manuscript and Itsuko Nakano for technical help.
PY - 2005/11/9
Y1 - 2005/11/9
N2 - In this study, we examined the cellular responses of stathmin-related proteins in the rat retina following optic nerve (ON) axotomy. To examine the distribution of stathmin-related gene products, we performed semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization (ISH) and immunohistochemical analyses. Retrograde labeling using a fluorescein tracer, fluorogold (FG), was used for the identification of retinal ganglion cells (RGCs). RT-PCR and ISH analyses indicated that the expression of RB3 was specifically increased in the ganglion cell layer (GCL) comparing to other members of stathmin-related gene family examined 3 days following the ON axotomy. When brain-derived neurotrophic factor was administrated intravitreouslly, the induction of RB3 mRNA sustained up to 7 days after axotomy, although the peak induction level was unchanged. In contrast, ciliary neurotrophic factor (CNTF) administration increased the peak level of RB3 mRNA induction significantly at 3 days after axotomy. Immunohistochemistry in combination with the retrograde labeling of axotomized cells by FG revealed that RB3 was increased following axotomy in FG-labeled RGCs. These data suggest that RB3 is the unique response protein in the stathmin-related proteins following ON axotomy and the induced RB3 may play a critical role in the CNTF-induced response on the axotomized RGCs, e.g. axonal regeneration and/or neuroprotection.
AB - In this study, we examined the cellular responses of stathmin-related proteins in the rat retina following optic nerve (ON) axotomy. To examine the distribution of stathmin-related gene products, we performed semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization (ISH) and immunohistochemical analyses. Retrograde labeling using a fluorescein tracer, fluorogold (FG), was used for the identification of retinal ganglion cells (RGCs). RT-PCR and ISH analyses indicated that the expression of RB3 was specifically increased in the ganglion cell layer (GCL) comparing to other members of stathmin-related gene family examined 3 days following the ON axotomy. When brain-derived neurotrophic factor was administrated intravitreouslly, the induction of RB3 mRNA sustained up to 7 days after axotomy, although the peak induction level was unchanged. In contrast, ciliary neurotrophic factor (CNTF) administration increased the peak level of RB3 mRNA induction significantly at 3 days after axotomy. Immunohistochemistry in combination with the retrograde labeling of axotomized cells by FG revealed that RB3 was increased following axotomy in FG-labeled RGCs. These data suggest that RB3 is the unique response protein in the stathmin-related proteins following ON axotomy and the induced RB3 may play a critical role in the CNTF-induced response on the axotomized RGCs, e.g. axonal regeneration and/or neuroprotection.
KW - Axotomy
KW - Growth associate protein
KW - Regeneration
KW - Retinal ganglion cell
KW - SCG10
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U2 - 10.1016/j.brainres.2005.08.055
DO - 10.1016/j.brainres.2005.08.055
M3 - Article
C2 - 16256088
AN - SCOPUS:27744562565
SN - 0006-8993
VL - 1061
SP - 97
EP - 106
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -