Selective suppression of stress-activated protein kinase pathway by protein phosphatase 2C in mammalian cells

Masahito Hanada, Takayasu Kobayashi, Motoko Ohnishi, Shoko Ikeda, Hong Wang, Koji Katsura, Yuchio Yanagawa, Akira Hiraga, Ryunosuke Kanamaru, Shinri Tamura

Research output: Contribution to journalArticlepeer-review

91 Citations (Scopus)

Abstract

Protein phosphatase 2Cα (PP2Cα) or PP2Cβ-1 expressed in COS7 cells suppressed anisomycin- and NaCl-enhanced phosphorylations of p38 co-expressed in the cells. PP2Cα or PP2Cβ-1 expression also suppressed both basal and stress-enhanced phosphorylations of MKK3b and MKK6b, which are upstream protein kinases of p38, and of MKK4, which is one of the major upstream protein kinases of JNK. Basal activity of MKK7, another upstream protein kinase of JNK, was also suppressed by PP2Cα or PP2Cβ-1 expression. However, basal as well as serum-activated phosphorylation of MKK1a, an upstream protein kinase of ERKs, was not affected by PP2Cβ or PP2Cβ-1. A catalytically inactive mutant of PP2Cβ-1 further enhanced the NaCl-stimulated phosphorylations of MMK3b, MKK4 and MKK6b, suggesting that this mutant PP2Cβ-1 works as a dominant negative form. These results suggest that PP2C selectively inhibits the SAPK pathways through suppression of MKK3b, MKK4, MKK6b and MKK7 activities in mammalian cells. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)172-176
Number of pages5
JournalFEBS Letters
Volume437
Issue number3
DOIs
Publication statusPublished - 1998 Oct 23
Externally publishedYes

Keywords

  • Protein phosphatase 2C
  • Stress-activated protein kinase signal pathway

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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