Selective regulation of antigen-specific IgE response by cyclic AMP level in murine lymphocytes

Masaki Hikida, Toshiyuki Takai, Hitoshi Ohmori

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have reported that prostaglandin E2 (PGE2) is a selective stimulator of the antigen-specific IgE response [6]. Because PGE2 is known to elevate intracellular cAMP, we investigated the regulatory role of cAMP in the production of antigen-specific IgE. Anti-TNP IgE response was induced by stimulating TNP-KLH-primed BALB/c spleen cells with the same antigen in vitro. Addition of 10-100 μM dibutyryl cAMP (DBcAMP) to the lymphocyte culture resulted in a 2-3-fold increase in anti-TNP IgE response without affecting the production of anti-TNP IgG1 or IgM. Forskolin, a stimulator of adenylate cyclase, also specifically augmented the IgE response. In contrast, 2′,5′-dideoxyadenosine, an inhibitor of adenylate cyclase, suppressed IgE production in an isotype-specific manner. These results suggest that IgE synthesis can be selectively modulated by intracellular cAMP level. Enhancement of IgE production by DBcAMP was observed, particularly in highly primed spleen cells, suggesting that IgE-committed B cells are subjected to regulation by cAMP.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalImmunology Letters
Volume33
Issue number3
DOIs
Publication statusPublished - 1992 Aug
Externally publishedYes

Keywords

  • Cyclic AMP
  • IgE response
  • Murine lymphocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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