Selective pairing and fusion of cell-sized liposomes using round-tip dielectrophoretic tweezer

Shotaro Yoshida, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we developed an improved dielectrophoretic (DEP) tweezer and utilized it to selective pairing and fusion of cell-sized liposomes. We patterned parylene insulation layer on the DEP tweezer to avoid electroosmotic flow, and put fluorescent-gel inside it to improve visibility. We also fabricated a liposome fusion device which has patterned SU-8 traps and cupper (Cu) electrodes, and demonstrated selective liposome pairing using the DEP tweezer and fusion of the liposomes. We believe our method will be useful in vesicle fusion assay for selective transfer of vesicle contents to another vesicle or biological cells.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages983-984
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020 Jan 1
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

Keywords

  • Dielectrophoresis
  • Electrode
  • Electrofusion
  • Liposome
  • Micromanipulation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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