Selective nitrosyl group transfer reaction to cytidine using oligonucleotides bearing S-nitrosothioguanosine.

Fumi Nagatsugi, Shizuka Nakayama, Monsur Md Ali, Shigeki Sasaki

Research output: Contribution to journalArticlepeer-review

Abstract

Nitric oxide (NO) is an important endogenous regulatory molecule, and conversely, it is also known to be mutagenic because of its ability of nitrosylation and following deamination of nucleobases. In this study, we aimed at developing a useful tool for nitrosylation to a specific site of DNA by the use of ODN 2 incorporating S-nitroso thioguanosine. It has been demonstrated that the S-NO containing ODN 2 exhibits rapid and specific NO-transfer reaction to its complementary ODN 5 having dC or d(m)C at the target site. Thus, we have established an innovative method for the highly efficient and selective transfer of NO to cytidine and 5-methylcytidine.

Original languageEnglish
Pages (from-to)23-24
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number48
Publication statusPublished - 2004
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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