Selective labeling of proteins on living cell membranes using fluorescent nanodiamond probes

Shingo Sotoma, Jun Iimura, Ryuji Igarashi, Koichiro M. Hirosawa, Hidenori Ohnishi, Shin Mizukami, Kazuya Kikuchi, Takahiro K. Fujiwara, Masahiro Shirakawa, Hidehito Tochio

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The impeccable photostability of fluorescent nanodiamonds (FNDs) is an ideal property for use in fluorescence imaging of proteins in living cells. However, such an application requires highly specific labeling of the target proteins with FNDs. Furthermore, the surface of unmodified FNDs tends to adsorb biomolecules nonspecifically, which hinders the reliable targeting of proteins with FNDs. Here, we combined hyperbranched polyglycerol modification of FNDs with the -lactamase-tag system to develop a strategy for selective imaging of the protein of interest in cells. The combination of these techniques enabled site-specific labeling of Interleukin-18 receptor alpha chain, a membrane receptor, with FNDs, which eventually enabled tracking of the diffusion trajectory of FND-labeled proteins on the membrane surface.

Original languageEnglish
Article number56
JournalNanomaterials
Volume6
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1
Externally publishedYes

Keywords

  • Membrane protein
  • Nanodiamond
  • Nitrogen-vacancy center
  • Polyglycerol
  • β-lactamase tag

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

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