Selective killing of HIV-infected cells by anti-gp120 immunotoxins

S. Matsushita, A. Koito, Y. Maeda, T. Hattori, K. Takatsuki

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Either ricin A chain (RAC) or Pseudomonas exotoxin (PE) was conjugated with a murine monoclonal antibody (0.5β) directed against an external evelope glycoprotein (gp120) of human immunodeficiency virus (HIV). Effects of the immunotoxins produced against infected cells were evaluated. Selective inhibition of the proliferation and killing of chronically HIV infected cells were observed in the presence of the immunotoxins. To determine the feasibility of the immunotoxins against the infected cells in seropositive subjects, we attempted to detect gp120-bearing cells in peripheral blood mononuclear cells (PBM) by cytofluorography. Cells in the monocyte/macrophage region of 2 of 10 PBM samples from HIV-infected individuals were found to react with 0.5β (18.1% and 12.8%). Furthermore, the cell population which was reactive with 0.5β was also susceptible to RAC conjugated with 0.5β. These results suggest that the strategy of using anti-gp120 immunotoxin to eliminate HIV-infected cells may be feasible in infected individuals.

Original languageEnglish
Pages (from-to)193-203
Number of pages11
JournalAIDS Research and Human Retroviruses
Volume6
Issue number2
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Immunology
  • Virology

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