Selective inactivation of elongation factor-2 (EF-2) in free adipocytes obtained by treating Sarcophaga larval fat bodies with chymotrypsin

Hajime Okuyama, Shoichiro Kurata, Ko Ichi Homma, Shunji Natori

Research output: Contribution to journalArticlepeer-review

Abstract

The molecular mechanism(s) involved in the selective cessation of protein synthesis in free adipocytes obtained by treating fat bodies of Sarcophaga larvae with chymotrypsin were investigated (Okuyama, H., Kurata, S., Natori, S., 1994. Insect Biochem. Mol. Biol. 24, 525-530). Ribosomes prepared from these adipocytes were normal, but the activity of soluble factor(s) needed for protein synthesis was greatly reduced compared with the control fat bodies. From experiments using diphtheria toxin, we concluded that EF-2, but not other factors, was selectively inactivated in these free adipocytes.

Original languageEnglish
Pages (from-to)301-307
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume28
Issue number5-6
DOIs
Publication statusPublished - 1998 May
Externally publishedYes

Keywords

  • Adipocyte
  • EF-2
  • Fat body
  • Protein synthesis
  • Sarcophaga peregrina
  • Tissue remodeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

Fingerprint Dive into the research topics of 'Selective inactivation of elongation factor-2 (EF-2) in free adipocytes obtained by treating Sarcophaga larval fat bodies with chymotrypsin'. Together they form a unique fingerprint.

Cite this