Selective formation of a complex of polyadenylate with an impurity in higher plant nucleic acid preparations

Takeshi Nitta, Hideki Takahashi, Koh Kobayashi, Keisuke Fukuei

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1 Citation (Scopus)

Abstract

Phenol-preparations of higher plant nucleic acids generally resulted in severe contamination by an impurity bound selectively to synthetic polyadenylate (poly(A)). The poly(A) was rapidly bound into a form that was resistant to RNase T2. The impurity sedimented at about 14 S in a sucrose density gradient and had a bouyant density of 1.61 g/cm3 resembling that of polysaccharide. Natural poly(A)-containing RNA also was bound effectively to this impurity.

Original languageEnglish
Pages (from-to)135-144
Number of pages10
JournalPlant Science Letters
Volume34
Issue number1-2
DOIs
Publication statusPublished - 1984 Jan 1
Externally publishedYes

Keywords

  • Contaminant
  • Hydrophobic binding of poly(A)
  • Poly(A)-containing mRNA
  • Proteoglycan
  • Purification of mRNA

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