Selective cessation of protein synthesis in adipocytes produced by dissociating Sarcophaga larval fat body with chymotrypsin

Hajime Okuyama, Shoichiro Kurata, Shunji Natori

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Disintegration of the larval fat body of Sarcophaga, which takes place in the early pupal stage was mimicked by digesting the fat body with chymotrypsin in vitro. The resulting free adipocytes produced in vitro were found to have no activity to synthesize protein. However, their viability, RNA synthesis, amino acid uptake and mRNA content were similar to those of cells in the fat body treated without chymotrypsin. These results suggest that selective cessation of protein synthesis is a prerequisite for remodeling the fat body during metamorphosis.

Original languageEnglish
Pages (from-to)525-530
Number of pages6
JournalInsect Biochemistry and Molecular Biology
Volume24
Issue number5
DOIs
Publication statusPublished - 1994 May
Externally publishedYes

Keywords

  • Adipocytes
  • Fat body decomposition
  • Metamorphosis
  • Protein synthesis
  • Sarcophaga peregrina

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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