Selective cell fusion method by laser device (Examination of hybridomas' proliferation potency and function)

H. Itagaki, H. Doi, N. Ohkohchi, S. Satomi, S. Satou, Y. Taguchi

Research output: Contribution to journalArticlepeer-review

Abstract

We developed a new cell fusion method which was sterile, noncontact, and selective technique under the microscope, using the micro-processing device by LASER. By this technique, we succeeded in fusing myeloma cell(SP2) and lymphocyte in mouse. We also defined the proliferation of the fused cells in HAT medium and the function of the fused cells in the Ouchterlony method, i.e. production of IgG. This method enable us to make hybrydomas from very small number of cells and to fuse target cells selectively. This method is applicable to fuse cells which are difficult to be fused by conventional methods.

Original languageEnglish
Pages (from-to)762-766
Number of pages5
JournalJapanese Journal of Artificial Organs
Volume26
Issue number3
Publication statusPublished - 1997

Keywords

  • Ouchterlony method
  • cell fusion
  • hybridoma
  • microprocessing
  • myeloma cell(SP2)

ASJC Scopus subject areas

  • Biophysics

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