Selective and specific cleavage of the D1 and D2 proteins of Photosystem II by exposure to singlet oxygen: Factors responsible for the susceptibility to cleavage of the proteins

Katsuhiko Okada, Masahiko Ikeuchi, Naoki Yamamoto, Taka Aki Ono, Mitsue Miyao

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

Exposure of isolated Photosystem II (PS II) complexes to singlet oxygen (1O2) results in cleavage of the D1 protein to specific fragments as does illumination with strong light (Mishra, N.P. and Ghanotakis, D.F. (1994) Biochim. Biophys. Acta 1187, 296-300). We reexamined the effects of 1O2, generated by the photosensitizing reaction of rose bengal, on proteins of the PS II complexes. It was found that the D1 protein and also the D2 protein were selectively cleaved into specific fragments as under strong illumination. This observation suggests that only the D1 and D2 proteins have amino acid sequences that are cleavable after attack by 1O2. These two proteins were almost equally susceptible to cleavage by 1O2. By contrast, when the PS II complexes that had been solubilized with SDS were exposed to 1O2, no distinct fragments of the D2 protein were detected, while the D1 protein was cleaved to specific fragments, though the yield of fragments was about half of that obtained from the intact PS II complexes. These results imply that the conformation of proteins is crucial for the specific cleavage by 1O2, and that only the D1 protein could retain the conformation required for the cleavage, though partly, after solubilization with SDS.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1274
Issue number1-2
DOIs
Publication statusPublished - 1996 May 20

Keywords

  • Active oxygen
  • D1 protein
  • Photoinhibition
  • Photosynthesis
  • Photosystem II
  • Singlet oxygen

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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