TY - JOUR
T1 - Selective accumulation of PpIX and photodynamic effect after aminolevulinic acid treatment of human adenomyosis xenografts in nude mice
AU - Suzuki-Kakisaka, Haruka
AU - Murakami, Takashi
AU - Hirano, Toru
AU - Terada, Yukihiro
AU - Yaegashi, Nobuo
AU - Okamura, Kunihiro
PY - 2008/10
Y1 - 2008/10
N2 - Objective: To evaluate the effect of photodynamic therapy with aminolevulinic acid (ALA) on human adenomyosis xenografts in a mouse model. Design: Human adenomyosis tissues were implanted SC into nude mice. We measured 5-aminolevulinic acid pharmacokinetics in these mice by analyzing tissue sections 1 to 6 hours after intraperitoneal administration. Twenty-four hours after photodynamic therapy, we evaluated tissue morphologic features. Setting: Department of obstetrics and gynecology at a university hospital in Japan. Patient(s): Immunodeficient mice. Tissue grafts were taken from women with adenomyosis attending a university hospital. Intervention(s): Photodynamic treatment. Main Outcome Measure(s): Peak fluorescence after intraperitoneal ALA administration and tissue histological changes 24 hours after photodynamic therapy. Result(s): Peak fluorescence was observed 3 hours after intraperitoneal administration. Histological studies revealed decreased numbers of epithelial and stromal cells in adenomyosis models after therapy. Conclusion(s): Photodynamic therapy with ALA caused extensive cell death in human adenomyosis tissues implanted into nude mice. Photodynamic treatment using ALA is a potential treatment for patients with adenomyosis uteri.
AB - Objective: To evaluate the effect of photodynamic therapy with aminolevulinic acid (ALA) on human adenomyosis xenografts in a mouse model. Design: Human adenomyosis tissues were implanted SC into nude mice. We measured 5-aminolevulinic acid pharmacokinetics in these mice by analyzing tissue sections 1 to 6 hours after intraperitoneal administration. Twenty-four hours after photodynamic therapy, we evaluated tissue morphologic features. Setting: Department of obstetrics and gynecology at a university hospital in Japan. Patient(s): Immunodeficient mice. Tissue grafts were taken from women with adenomyosis attending a university hospital. Intervention(s): Photodynamic treatment. Main Outcome Measure(s): Peak fluorescence after intraperitoneal ALA administration and tissue histological changes 24 hours after photodynamic therapy. Result(s): Peak fluorescence was observed 3 hours after intraperitoneal administration. Histological studies revealed decreased numbers of epithelial and stromal cells in adenomyosis models after therapy. Conclusion(s): Photodynamic therapy with ALA caused extensive cell death in human adenomyosis tissues implanted into nude mice. Photodynamic treatment using ALA is a potential treatment for patients with adenomyosis uteri.
KW - 5-aminoleuvulinic acid
KW - Photodynamic therapy
KW - adenomyosis
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U2 - 10.1016/j.fertnstert.2007.09.001
DO - 10.1016/j.fertnstert.2007.09.001
M3 - Article
C2 - 18462731
AN - SCOPUS:52949096880
VL - 90
SP - 1523
EP - 1527
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 4 SUPPL.
ER -