Selection of in vitro-matured porcine oocytes based on localization patterns of lipid droplets to evaluate developmental competence

Kou Hiraga, Yumi Hoshino, Kentaro Tanemura, Eimei Sato

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Localization patterns of lipid droplets in the cytoplasm of porcine oocytes were evaluated as a novel marker for in vitro maturation (IVM) of oocytes with high developmental competence. Porcine oocytes were cultured in TCM-199, which is a complete synthetic medium, for 44 h at 38.5 C. Localization patterns were divided into 2 classes: lipid droplets localized uniformly in the whole cytoplasm (class I) and those that were centrally located (class II). After IVM in TCM-199, 60% of matured oocytes exhibited the class II pattern. To investigate the relation between the distribution of lipid droplets and the developmental rate of the oocyte, the developmental rates of class I and class II oocytes were compared after in vitro fertilization (IVF). Class II oocytes showed a significantly higher rate of blastocyst development than class I oocytes. These results suggest that porcine oocytes with high developmental competence can be selected based on the localization patterns of lipid droplets.

Original languageEnglish
Pages (from-to)405-408
Number of pages4
JournalJournal of Reproduction and Development
Volume59
Issue number4
DOIs
Publication statusPublished - 2013

Keywords

  • Lipid droplet
  • Novel marker
  • Porcine oocyte

ASJC Scopus subject areas

  • Animal Science and Zoology

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