Selection of cellular genetic markers for the detection of infectious poliovirus

D. Sano, M. Tazawa, M. Inaba, S. Kadoya, R. Watanabe, T. Miura, M. Kitajima, S. Okabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aims: Cellular responses of an established cell line from human intestinal epithelial cells (INT-407 cells) against poliovirus (PV) infections were investigated in order to find cellular genetic markers for infectious PV detection. Methods and Results: Gene expression profile of INT-407 cells was analysed by DNA microarray technique when cells were infected with poliovirus 1 (PV1) (sabin) at multiplicity of infection of 10−3 and incubated for 12 h. Poliovirus infection significantly altered the gene expressions of two ion channels, KCNJ4 and SCN7A. The expression profile of KCNJ4 gene was further investigated by real-time RT-qPCR, and it was found that KCNJ4 gene was significantly regulated at 24 h postinfection of PV1. Conclusions: KCNJ4 gene, coding a potassium channel protein, is proposed as a cellular genetic marker for infectious PV detection. Significance and Impact of the Study: This is the first study to show the availability of cellular responses to detect infectious PV. The selection of cellular genetic markers for infectious viruses using DNA microarray and RT-qPCR can be applicable for the other enteric viruses.

Original languageEnglish
Pages (from-to)1001-1007
Number of pages7
JournalJournal of Applied Microbiology
Volume124
Issue number4
DOIs
Publication statusPublished - 2018 Apr

Keywords

  • DNA microarray
  • cellular responses
  • genetic markers
  • infectious poliovirus
  • ion channels

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Selection of cellular genetic markers for the detection of infectious poliovirus'. Together they form a unique fingerprint.

  • Cite this

    Sano, D., Tazawa, M., Inaba, M., Kadoya, S., Watanabe, R., Miura, T., Kitajima, M., & Okabe, S. (2018). Selection of cellular genetic markers for the detection of infectious poliovirus. Journal of Applied Microbiology, 124(4), 1001-1007. https://doi.org/10.1111/jam.13621