Selection of cellular genetic markers for the detection of infectious poliovirus

D. Sano, M. Tazawa, M. Inaba, S. Kadoya, R. Watanabe, T. Miura, M. Kitajima, S. Okabe

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Aims: Cellular responses of an established cell line from human intestinal epithelial cells (INT-407 cells) against poliovirus (PV) infections were investigated in order to find cellular genetic markers for infectious PV detection. Methods and Results: Gene expression profile of INT-407 cells was analysed by DNA microarray technique when cells were infected with poliovirus 1 (PV1) (sabin) at multiplicity of infection of 10−3 and incubated for 12 h. Poliovirus infection significantly altered the gene expressions of two ion channels, KCNJ4 and SCN7A. The expression profile of KCNJ4 gene was further investigated by real-time RT-qPCR, and it was found that KCNJ4 gene was significantly regulated at 24 h postinfection of PV1. Conclusions: KCNJ4 gene, coding a potassium channel protein, is proposed as a cellular genetic marker for infectious PV detection. Significance and Impact of the Study: This is the first study to show the availability of cellular responses to detect infectious PV. The selection of cellular genetic markers for infectious viruses using DNA microarray and RT-qPCR can be applicable for the other enteric viruses.

Original languageEnglish
Pages (from-to)1001-1007
Number of pages7
JournalJournal of Applied Microbiology
Issue number4
Publication statusPublished - 2018 Apr


  • DNA microarray
  • cellular responses
  • genetic markers
  • infectious poliovirus
  • ion channels

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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