Selection of 5-methyltryptophan-resistant asparagus with ethyl methanesulfonate treatment

T. Sonoda, Y. Hayashi, A. Kanno, T. Kameya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Immediately after pollination female flowers of asparagus plants were treated with ethyl methanesulfonate for selection of mutants resistant to 5-methyltryptophan (5MT), and seeds (M1) were obtained after 3 months. The M1 seeds were lighter and snowed a lower germination rate respect to control seeds. Various morphological mutations were observed in the Ml seedlings and many of them had longer cladophylls and shorter internodes than control plants. Meristems originating from the Ml plants were cultured on medium containing 5MT. Selection based on the shoot growth index was carried out after 3 weeks, and a few 5MT-resistant lines were selected among culture lines.

Original languageEnglish
Title of host publicationProceedings of the Eleventh International Asparagus Symposium
PublisherInternational Society for Horticultural Science
Pages333-335
Number of pages3
ISBN (Print)9789066056701
DOIs
Publication statusPublished - 2008 Jan

Publication series

NameActa Horticulturae
Volume776
ISSN (Print)0567-7572

Keywords

  • 5-methyltryptophan-resistant
  • Asparagus officinalis L.
  • Ethyl methanesulfonate
  • Mutant
  • Selection

ASJC Scopus subject areas

  • Horticulture

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  • Cite this

    Sonoda, T., Hayashi, Y., Kanno, A., & Kameya, T. (2008). Selection of 5-methyltryptophan-resistant asparagus with ethyl methanesulfonate treatment. In Proceedings of the Eleventh International Asparagus Symposium (pp. 333-335). (Acta Horticulturae; Vol. 776). International Society for Horticultural Science. https://doi.org/10.17660/actahortic.2008.776.43