Seasonal changes in the spermatogenesis of the large Japanese field mice (Apodemus speciosus) controlled by proliferation and apoptosis of germ cells

Jun Ito, Kanna Meguro, Kazuki Komatsu, Takuya Ohdaira, Rina Shoji, Takahisa Yamada, Satoshi Sugimura, Yohei Fujishima, Akifumi Nakata, Manabu Fukumoto, Tomisato Miura, Hideaki Yamashiro

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The aim of this study was to investigate the proliferation and apoptosis of male germ cells during the seasonal reproductive cycle of the large Japanese field mice (Apodemus speciosus). Male mice residing in their natural habitat were captured in Niigata, Japan. Testis sections were stained with haematoxylin and eosin, and mitotic male germ cells were identified using immunofluorescence staining for proliferating cell nuclear antigen (PCNA). Apoptosis was analysed using terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling (TUNEL) assay. The phases of spermatogenesis during the seasonal reproductive cycle were classified as active, transitional, and inactive based on the diameter of the seminiferous tubules. The number of PCNA-positive germ cells was less during the inactive than other phases. The percentage of TUNEL-positive germ cells per seminiferous tubule was greater during the inactive than active and transitional phases. Spermatogenesis during the seasonal reproductive cycle is controlled by proliferation and apoptosis in male germ cells. This species of undomesticated mice could be used as an animal model to study spermatogenesis as a valuable indicator of the effects of ecological and anthropogenic factors on animal reproduction.

Original languageEnglish
Article number106288
JournalAnimal Reproduction Science
Volume214
DOIs
Publication statusPublished - 2020 Mar

Keywords

  • Apodemus speciosus
  • Seasonal reproductive cycle
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

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