TY - JOUR
T1 - Seasonal changes in the spermatogenesis of the large Japanese field mice (Apodemus speciosus) controlled by proliferation and apoptosis of germ cells
AU - Ito, Jun
AU - Meguro, Kanna
AU - Komatsu, Kazuki
AU - Ohdaira, Takuya
AU - Shoji, Rina
AU - Yamada, Takahisa
AU - Sugimura, Satoshi
AU - Fujishima, Yohei
AU - Nakata, Akifumi
AU - Fukumoto, Manabu
AU - Miura, Tomisato
AU - Yamashiro, Hideaki
N1 - Funding Information:
This research was supported by the Japan Atomic Energy Agency (JAEA) by the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT). This work was also supported by the J apan Society for the Promotion of Science (JSPS). Appendix A
Publisher Copyright:
© 2020
PY - 2020/3
Y1 - 2020/3
N2 - The aim of this study was to investigate the proliferation and apoptosis of male germ cells during the seasonal reproductive cycle of the large Japanese field mice (Apodemus speciosus). Male mice residing in their natural habitat were captured in Niigata, Japan. Testis sections were stained with haematoxylin and eosin, and mitotic male germ cells were identified using immunofluorescence staining for proliferating cell nuclear antigen (PCNA). Apoptosis was analysed using terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling (TUNEL) assay. The phases of spermatogenesis during the seasonal reproductive cycle were classified as active, transitional, and inactive based on the diameter of the seminiferous tubules. The number of PCNA-positive germ cells was less during the inactive than other phases. The percentage of TUNEL-positive germ cells per seminiferous tubule was greater during the inactive than active and transitional phases. Spermatogenesis during the seasonal reproductive cycle is controlled by proliferation and apoptosis in male germ cells. This species of undomesticated mice could be used as an animal model to study spermatogenesis as a valuable indicator of the effects of ecological and anthropogenic factors on animal reproduction.
AB - The aim of this study was to investigate the proliferation and apoptosis of male germ cells during the seasonal reproductive cycle of the large Japanese field mice (Apodemus speciosus). Male mice residing in their natural habitat were captured in Niigata, Japan. Testis sections were stained with haematoxylin and eosin, and mitotic male germ cells were identified using immunofluorescence staining for proliferating cell nuclear antigen (PCNA). Apoptosis was analysed using terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling (TUNEL) assay. The phases of spermatogenesis during the seasonal reproductive cycle were classified as active, transitional, and inactive based on the diameter of the seminiferous tubules. The number of PCNA-positive germ cells was less during the inactive than other phases. The percentage of TUNEL-positive germ cells per seminiferous tubule was greater during the inactive than active and transitional phases. Spermatogenesis during the seasonal reproductive cycle is controlled by proliferation and apoptosis in male germ cells. This species of undomesticated mice could be used as an animal model to study spermatogenesis as a valuable indicator of the effects of ecological and anthropogenic factors on animal reproduction.
KW - Apodemus speciosus
KW - Seasonal reproductive cycle
KW - Spermatogenesis
KW - Testis
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U2 - 10.1016/j.anireprosci.2020.106288
DO - 10.1016/j.anireprosci.2020.106288
M3 - Article
C2 - 32087913
AN - SCOPUS:85077999993
VL - 214
JO - Animal Reproduction Science
JF - Animal Reproduction Science
SN - 0378-4320
M1 - 106288
ER -