TY - JOUR
T1 - Seagrass as a potential source of natural antioxidant and anti-inflammatory agents
AU - Yuvaraj, N.
AU - Kanmani, P.
AU - Satishkumar, R.
AU - Paari, A.
AU - Pattukumar, V.
AU - Arul, V.
N1 - Funding Information:
This work was supported by a grant from Department of Biotechnology (DBT), New Delhi, India. The authors declare that there are no other conflicts of interest.
PY - 2012/4
Y1 - 2012/4
N2 - Context: Halophila spp. is a strong medicine against malaria and skin diseases and is found to be very effective in early stages of leprosy. Seagrasses are nutraceutical in nature and therefore of importance as food supplements. Objective: The antibacterial, antioxidant, and anti-inflammatory activities of Halophila ovalis R. Br. Hooke (Hydrocharitaceae) methanol extract were investigated and the chemical constituents of purified fractions were analyzed. Materials and methods: Plant materials were collected from Pondicherry coastal line, and antimicrobial screening of crude extract, and purified fractions was carried out by the disc diffusion method and the minimum inhibitory concentration (MICs) of the purified fractions and reference antibiotics were determined by microdilution method. Antioxidant and anti-inflammatory activities were investigated in vitro. Chemical constituents of purified fractions V and VI were analyzed by gas chromatographymass spectrometry (GCMS), and the phytochemicals were quantitatively determined. Results: Methanol extract inhibited the growth of Bacillus cereus at a minimum inhibitory concentration of 50 g/mL and other Gram-negative pathogens at 75 g/ml, except Vibrio vulnificus. Reducing power and total antioxidant level increased with increasing extract concentration. H. ovalis exhibited strong scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals at IC 50 of 0.13 and 0.65mg/mL, respectively. Methanol extract of H. ovalis showed noticeable anti-inflammatory activity at IC 50 of 78.72 g/mL. The GCMS analysis of H. ovalis revealed the presence of triacylglycerols as major components in purified fractions. Quantitative analysis of phytochemicals revealed that phenols are rich in seagrass H. ovalis. Discussion and conclusion: These findings demonstrated that the methanol extract of H. ovalis exhibited appreciable antibacterial, noticeable antioxidant, and anti-inflammatory activities, and thus could be use as a potential source for natural health products.
AB - Context: Halophila spp. is a strong medicine against malaria and skin diseases and is found to be very effective in early stages of leprosy. Seagrasses are nutraceutical in nature and therefore of importance as food supplements. Objective: The antibacterial, antioxidant, and anti-inflammatory activities of Halophila ovalis R. Br. Hooke (Hydrocharitaceae) methanol extract were investigated and the chemical constituents of purified fractions were analyzed. Materials and methods: Plant materials were collected from Pondicherry coastal line, and antimicrobial screening of crude extract, and purified fractions was carried out by the disc diffusion method and the minimum inhibitory concentration (MICs) of the purified fractions and reference antibiotics were determined by microdilution method. Antioxidant and anti-inflammatory activities were investigated in vitro. Chemical constituents of purified fractions V and VI were analyzed by gas chromatographymass spectrometry (GCMS), and the phytochemicals were quantitatively determined. Results: Methanol extract inhibited the growth of Bacillus cereus at a minimum inhibitory concentration of 50 g/mL and other Gram-negative pathogens at 75 g/ml, except Vibrio vulnificus. Reducing power and total antioxidant level increased with increasing extract concentration. H. ovalis exhibited strong scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals at IC 50 of 0.13 and 0.65mg/mL, respectively. Methanol extract of H. ovalis showed noticeable anti-inflammatory activity at IC 50 of 78.72 g/mL. The GCMS analysis of H. ovalis revealed the presence of triacylglycerols as major components in purified fractions. Quantitative analysis of phytochemicals revealed that phenols are rich in seagrass H. ovalis. Discussion and conclusion: These findings demonstrated that the methanol extract of H. ovalis exhibited appreciable antibacterial, noticeable antioxidant, and anti-inflammatory activities, and thus could be use as a potential source for natural health products.
KW - Antibacterial
KW - GCMS
KW - Halophila ovalis
KW - MIC
KW - Triacylglycerols
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U2 - 10.3109/13880209.2011.611948
DO - 10.3109/13880209.2011.611948
M3 - Article
C2 - 22129224
AN - SCOPUS:84858275729
VL - 50
SP - 458
EP - 467
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
SN - 1388-0209
IS - 4
ER -
