TY - JOUR
T1 - Screening of wound-responsive genes identifies an immediate-early expressed gene encoding a highly charged protein in mechanically wounded tobacco plants
AU - Hara, Kojiro
AU - Yagi, Mitsue
AU - Koizumi, Nozomu
AU - Kusano, Tomonobu
AU - Sano, Hiroshi
N1 - Funding Information:
The authors thank Drs. T. Hakoshima and M. Shirakawa (Nara Institute of Science and Technology) for helpful discussions, Drs. Y. Ohashi (National Institute of Agrobiological Resources) and A. Watanabe (University of Tokyo) for supplying the probes and Dr. David B. Douglas for critical reading of the manuscript. This work was partly supported by grants from the "Research for the Future" Program (JSPS-RFTF96R160012) of the Japan Society for the Promotion of Science, and from the Enhancement of Center-of-Excellence, Special Coordination Funds for Promoting Science and Technology, Science and Technology Agency, Japan. KH is a recipient of a Research Fellowship for Young Scientists from the Japan Society for the Promotion of Science.
PY - 2000
Y1 - 2000
N2 - In order to identify genes that are temporally and spatially regulated during wound response, a cDNA population in mechanically wounded tobacco leaves was screened by the fluorescence differential display method. Of 28 clones initially identified to have altered levels of transcripts within 3 h of wounding, eight were characterized. Although each clone showed a unique pattern of transcript accumulation, one distinct clone was further characterized because of its immediate-early response. Its transcripts began to accumulate 10 rain after wounding, reached a maximum level within 1 h and disappeared after 2 h. The response, which occurred repeatably and systemically, was observed by the treatment with propionic acid or erythrosin B, indicating that cytosolic acidification could be one of the signals for immediate-early response of this gene. The cDNA encodes a polypeptide of 513 amino acids with a relative molecular mass of 60,952. The putative polypeptlde is rich in lysine (K), glutamic acid (E) and aspartic acid (D), which constitute up to 70% of total amino acids, and was therefore designated as KED. The KED polypeptide is composed of a highly hydrophilic N-terminal region and a relatively hydrophobic C-terminal region, suggesting that KED may function through electrostatic interactions with cellular components.
AB - In order to identify genes that are temporally and spatially regulated during wound response, a cDNA population in mechanically wounded tobacco leaves was screened by the fluorescence differential display method. Of 28 clones initially identified to have altered levels of transcripts within 3 h of wounding, eight were characterized. Although each clone showed a unique pattern of transcript accumulation, one distinct clone was further characterized because of its immediate-early response. Its transcripts began to accumulate 10 rain after wounding, reached a maximum level within 1 h and disappeared after 2 h. The response, which occurred repeatably and systemically, was observed by the treatment with propionic acid or erythrosin B, indicating that cytosolic acidification could be one of the signals for immediate-early response of this gene. The cDNA encodes a polypeptide of 513 amino acids with a relative molecular mass of 60,952. The putative polypeptlde is rich in lysine (K), glutamic acid (E) and aspartic acid (D), which constitute up to 70% of total amino acids, and was therefore designated as KED. The KED polypeptide is composed of a highly hydrophilic N-terminal region and a relatively hydrophobic C-terminal region, suggesting that KED may function through electrostatic interactions with cellular components.
KW - Charged protein
KW - Fluorescence differential display
KW - Nicotiana tabacum
KW - Systemic response
KW - Wounding
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U2 - 10.1093/pcp/41.6.684
DO - 10.1093/pcp/41.6.684
M3 - Article
C2 - 10945337
AN - SCOPUS:0033924459
VL - 41
SP - 684
EP - 691
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 6
ER -