TY - JOUR
T1 - Screening of new antioxidant molecules using flow cytometry
AU - Ostrovidow, Serge Jacques Pierre
AU - Franck, Patricia
AU - Joseph, Delphine
AU - Martarello, Laurent
AU - Kirsch, Gilbert
AU - Belleville, Francine
AU - Nabet, Pierre
AU - Dousset, Brigitte
PY - 2000/5/30
Y1 - 2000/5/30
N2 - We present a flow cytometry technique to evaluate the antioxidative properties of molecules on living cells, using a stable murine-murine hybridoma (Mark 3) cell line routinely cultured. Using this technique, intracellular superoxide anions and peroxides were evaluated with dihydrorhodamine (DHR-123) and dichloroflourescein diacetate (DCFH-DA), respectively. When cells were first incubated for 10 min with either H2O2 or the xanthine (X)/xanthine oxidase (XO) system, this flow cytometric technique was capable of evaluating the oxidative stress on cells. Twenty-one new analogues of ellipticine were synthesized and tested for their antioxidative properties compared to vitamin E and Ebselen used as references. A good statistical reflection of the antioxidative activities of these molecules was achieved by analyzing 35 000 cells in each experiment. Among them, the selenated molecule 18 was found to be 10 times more active than Ebselen but 10 000 times less active than vitamin E. Moreover, eight compounds showed glutathione peroxidase-like activities.
AB - We present a flow cytometry technique to evaluate the antioxidative properties of molecules on living cells, using a stable murine-murine hybridoma (Mark 3) cell line routinely cultured. Using this technique, intracellular superoxide anions and peroxides were evaluated with dihydrorhodamine (DHR-123) and dichloroflourescein diacetate (DCFH-DA), respectively. When cells were first incubated for 10 min with either H2O2 or the xanthine (X)/xanthine oxidase (XO) system, this flow cytometric technique was capable of evaluating the oxidative stress on cells. Twenty-one new analogues of ellipticine were synthesized and tested for their antioxidative properties compared to vitamin E and Ebselen used as references. A good statistical reflection of the antioxidative activities of these molecules was achieved by analyzing 35 000 cells in each experiment. Among them, the selenated molecule 18 was found to be 10 times more active than Ebselen but 10 000 times less active than vitamin E. Moreover, eight compounds showed glutathione peroxidase-like activities.
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U2 - 10.1021/jm991019j
DO - 10.1021/jm991019j
M3 - Article
C2 - 10794693
AN - SCOPUS:0034069170
VL - 43
SP - 1762
EP - 1769
JO - Journal of Medicinal Chemistry
JF - Journal of Medicinal Chemistry
SN - 0022-2623
IS - 9
ER -