Screening of IgG-Fc binding peptides from milk protein using slide glass type-exclusive peptide array

Makoto Katayama, Tomoya Sugita, Ryuji Kato, Mina Okochi, Miyoko Matsushima, Tsutomu Kawabe, Tomokazu Takase, Yasuko Yoshida, Mitsuo Kawase, Hiroyuki Honda

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

IgG-Fc binding peptide which functions as ligand for IgG purification was screened by peptide array. When sera from milk allergy patient were applied to the milk protein-explosive peptide array, some 16-mer binding peptides were found from both positive sera and negative sera. When cellulose membrane type peptide array was used for identification of binding peptide sequence, octamer peptide, LLRLKKYK, was found to strongly bind to mouse IgG. Since the octamer peptide weakly bound with IgA, it was suggested that the peptide is an IgG-Fc specific binding peptide. Furthermore, it was also suggested that the IgG-Fc binding peptide is available for purification of IgG-Fc.

Original languageEnglish
Pages (from-to)546-550
Number of pages5
Journalkagaku kogaku ronbunshu
Volume37
Issue number6
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • Antibody
  • Binding
  • IgG-Fc region
  • Peptide
  • Peptide array
  • Purification

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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