Screening of DNA aptamers against multiple proteins in tissue.

Takahisa Noma, Kazunori Ikebukuro, Koji Sode, Takuya Ohkubo, Yuji Sakasegawa, Naomi Hachiya, Kiyotoshi Kaneko

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We report the development of a novel screening method for DNA aptamers against multiple proteins in the target tissue. Although a purified single protein is generally used in screening of aptamers at present, such a simultaneous selection would be very advantageous in efficiency and selectivity. We first carried out the screening in situ and it was suggested that aptamers against particular target proteins were enriched. We also developed another novel screening method named Aptamer Blotting based on protein separation by PAGE and its visualization with fluorescein labeled aptamers and it was demonstrated that this method allowed the simultaneous selection of specific aptamers against multiple proteins.

Original languageEnglish
Pages (from-to)357-358
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number49
DOIs
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Noma, T., Ikebukuro, K., Sode, K., Ohkubo, T., Sakasegawa, Y., Hachiya, N., & Kaneko, K. (2005). Screening of DNA aptamers against multiple proteins in tissue. Nucleic acids symposium series (2004), (49), 357-358. https://doi.org/10.1093/nass/49.1.357