Screening of DNA aptamer which binds to α-synuclein

Kaori Tsukakoshi, Ryuichi Harada, Koji Sode, Kazunori Ikebukuro

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

α-Synuclein is a native, unfolded protein that causes several neurodegenerative diseases such as dementia with Lewy bodies and Parkinson's disease. We have now identified the first DNA aptamers against α-synuclein using native PAGE applied to the SELEX method. We call this aptamer "M5-15"; it is the α-synuclein-bound aptamer and was isolated after four cycles of screening. M5-15 is composed of three stem-loop structures that may play an important role in the binding to α-synuclein. Moreover, M5-15 specifically binds to the α-synuclein monomer and oligomer. We expect that this aptamer will become a useful tool in α-synuclein analysis and diagnosis.

Original languageEnglish
Pages (from-to)643-648
Number of pages6
JournalBiotechnology Letters
Volume32
Issue number5
DOIs
Publication statusPublished - 2010 Feb 1
Externally publishedYes

Keywords

  • Alzheimer's disease
  • DNA aptamer
  • Lewy bodies
  • Parkinson's disease
  • α-Synuclein

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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