Screening for functional tumor suppressor activity in pancreatic cancer

Liviu Lefter, Viorel Scripcariu, Makoto Sunamura, Toru Furukawa, Seiki Matsuno, Mitsuo Oshimura, Vasile Drug, Cristian Dragomir, Akira Horii

Research output: Contribution to journalArticlepeer-review

Abstract

Pancreatic cancer has a well-known reputation as one of the leading causes of cancer deaths worldwide. Thus, acquisition of efficient approaches and markers for accurate detection at the earlier stages of the disease should be prioritized. We have been focusing on tumor suppressor genes (TSGs) activity in pancreatic cancer to find effective methods for its genetic diagnosis and/or treatment. In this study, we utilized the technique of micro-cell-mediated chromosome transfer (MMCT) to introduce a normal copy of human chromosome 18 individually into some pancreatic cancer cells. Subsequently, the tumorigenic ability of the resulting hybrids was assessed in vitro and in vivo. In vitro growth of the hybrid clones was significantly delayed as compared to the parental cells. This was paralleled by the hybrid cells promotion of invasive carcinomas in nude mice at a significantly lower rate and with a longer latency than the parental tumor cells. This study provides evidence that MMCT is an efficient tool for screening of tumor suppressor activity in pancreatic cancer. The functional data emerging from this study bring into sharp relief the implication of chromosome 18 as a putative location for new TSG(s), yet to be identified in this region.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalRomanian Journal of Gastroenterology
Volume13
Issue number3
Publication statusPublished - 2004 Sep

Keywords

  • Chromosome 18
  • Pancreatic cancer
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Gastroenterology

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