TY - JOUR
T1 - Scrapie removal using Planova® virus removal filters
AU - Tateishi, Jun
AU - Kitamoto, Tetsuyuki
AU - Mohri, Shirou
AU - Satoh, Sakae
AU - Sato, Tetsuo
AU - Shepherd, Ailsa
AU - Macnaughton, Malcolm R.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - As a possible method for reducing the risk of transmissible spongiform encephalopathy (TSE) infection, Planova® virus removal filters were tested for their ability to remove scrapie agent ME7. Albumin solution was spiked with high-titre ME7 and filtered through three different pore sizes of Planova® filters. Infectivity of the pre- and post-filtration samples was assayed in log dilutions by intracerebral inoculation into C57B1/6 mice. Filtration of albumin solution in the absence or presence of a detergent (Sarkosyl) with Planova® 35N (35±2 nm mean pore size) removed the contaminating scrapie agent with reduction factors of 4.93 log10 and 1.61 log10, respectively. Filtration, both in the absence and presence of detergent with Planova® 15N (15±2 nm mean pore size), and in the presence of detergent with Planova® 10N (9±2 nm mean pore size), showed high levels of scrapie reduction of >5.87 log10, >4.21 log10, and >3.80 log10, respectively, with no residual infectively detected in any of the filtrate samples. The effectiveness of Planova® 35N filtration for the removal of infectivity of this TSE agent is greatly reduced in the presence of a strong detergent, but Planova® filters with 15 nm or smaller pore size membranes can remove such infectivity at high reduction rates.
AB - As a possible method for reducing the risk of transmissible spongiform encephalopathy (TSE) infection, Planova® virus removal filters were tested for their ability to remove scrapie agent ME7. Albumin solution was spiked with high-titre ME7 and filtered through three different pore sizes of Planova® filters. Infectivity of the pre- and post-filtration samples was assayed in log dilutions by intracerebral inoculation into C57B1/6 mice. Filtration of albumin solution in the absence or presence of a detergent (Sarkosyl) with Planova® 35N (35±2 nm mean pore size) removed the contaminating scrapie agent with reduction factors of 4.93 log10 and 1.61 log10, respectively. Filtration, both in the absence and presence of detergent with Planova® 15N (15±2 nm mean pore size), and in the presence of detergent with Planova® 10N (9±2 nm mean pore size), showed high levels of scrapie reduction of >5.87 log10, >4.21 log10, and >3.80 log10, respectively, with no residual infectively detected in any of the filtrate samples. The effectiveness of Planova® 35N filtration for the removal of infectivity of this TSE agent is greatly reduced in the presence of a strong detergent, but Planova® filters with 15 nm or smaller pore size membranes can remove such infectivity at high reduction rates.
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U2 - 10.1006/biol.2001.0269
DO - 10.1006/biol.2001.0269
M3 - Article
C2 - 11482889
AN - SCOPUS:0034903140
VL - 29
SP - 17
EP - 25
JO - Journal of Biological Standardization
JF - Journal of Biological Standardization
SN - 1045-1056
IS - 1
ER -