Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis

Tetsuyuki Kitamoto, Kohtaro Hikita, Takatoshi Tashima, Jun Tateishi, Yuji Sato

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.

Original languageEnglish
Pages (from-to)459-465
Number of pages7
JournalBioscience Reports
Volume6
Issue number5
DOIs
Publication statusPublished - 1986 May 1
Externally publishedYes

Keywords

  • Alzheimer's disease
  • SAF
  • amyloid fibril protein
  • systemic amyloidosis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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