SCF β-TRCP promotes cell growth by targeting PR-Set7/Set8 for degradation

Zhiwei Wang, Xiangpeng Dai, Jiateng Zhong, Hiroyuki Inuzuka, Lixin Wan, Xiaoning Li, Lixia Wang, Xiantao Ye, Liankun Sun, Daming Gao, Lee Zou, Wenyi Wei

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The Set8/PR-Set7/KMT5a methyltransferase plays critical roles in governing transcriptional regulation, cell cycle progression and tumorigenesis. Although CRL4 Cdt2 was reported to regulate Set8 stability, deleting the PIP motif only led to partial resistance to ultraviolet-induced degradation of Set8, indicating the existence of additional E3 ligase(s) controlling Set8 stability. Furthermore, it remains largely undefined how DNA damage-induced kinase cascades trigger the timely destruction of Set8 to govern tumorigenesis. Here, we report that SCF β-TRCP earmarks Set8 for ubiquitination and degradation in a casein kinase I-dependent manner, which is activated by DNA-damaging agents. Biologically, both CRL4 Cdt2 and SCF β-TRCP -mediated pathways contribute to ultraviolet-induced Set8 degradation to control cell cycle progression, governing the onset of DNA damage-induced checkpoints. Therefore, like many critical cell cycle regulators including p21 and Cdt1, we uncover a tight regulatory network to accurately control Set8 abundance. Our studies further suggest that aberrancies in this delicate degradation pathway might contribute to aberrant elevation of Set8 in human tumours.

Original languageEnglish
Article number10185
JournalNature communications
Volume6
DOIs
Publication statusPublished - 2015 Dec 15
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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