Scavenging systems for reactive carbonyls in the cyanobacterium synechocystis sp. PCC 6803

Ginga Shimakawa, Mayumi Suzuki, Eriko Yamamoto, Akiko Nishi, Ryota Saito, Katsuhiko Sakamoto, Hiroshi Yamamoto, Amane Makino, Chikahiro Miyake

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

To elucidate the scavenging systems of sugar- and lipid-derived reactive carbonyls (RCs) in the cyanobacterium Synechocystis sp. PCC 6803 (S. 6803), we selected proteins from S. 6803 based on amino-acid (AA) sequence similarities with proteins from Arabidopsis thaliana, and characterized the properties of the GST-fusion proteins expressed. Slr0942 catalyzed the aldo-keto reductase (AKR) reaction scavenging mainly sugar-derived RCs, methylglyoxal (MG). Slr1192 is the medium-chain dehydrogenase/redutase (MDR). It catalyzed the AKR reaction scavenging several lipid-derived RCs, acrolein, propionaldehyde, and crotonaldehyde. Slr0315 is a short-chain dehydrogenase/redutase (SDR), and it catalyzed only the reduction of MG in the AKR reaction. Slr0381 catalyzed the conversion of hemithioacetal to S-lactoylglutahione (SLG) in the glyoxalase (GLX) 1 reaction. Sll1019 catalyzed the conversion of SLG to glutathione and lactate in the GLX2 reaction. GLX1 and GLX2 compose the glyoxalase system, which scavenges MG. These enzymes contribute to scavenging sugar- and lipid-derived RCs as scavenging systems.

Original languageEnglish
Pages (from-to)2441-2448
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number12
DOIs
Publication statusPublished - 2013

Keywords

  • Aldo-keto reductase (AKR)
  • Cyanobacteria
  • Glyoxalase (GLX)
  • Medium-chain dehydrogenase/ reductase (MDR)
  • Short-chain dehydrogenase/ reductase (SDR)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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