Scavenging or quenching effect of melanin on superoxide anion and singlet oxygen

Mika Tada, Masahiro Kohno, Yoshimi Niwano

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)


Although photoprotective properties of skin melanin have been well documented, a few studies on the effect of melanin on reactive oxygen species (ROS) generated by ultraviolet (UV) irradiation have been reported. To study the interaction of melanin with ROS, scavenging or quenching effect of melanin on O2.- and 1O2 was examined by electron spin resonance (ESR)-spin trapping methods and a spectrophotometric method, respectively. Melanin potently interacted with O2.- generated in a hypoxanthine (HPX)-xanthine oxidase (XOD) reaction, and with 1O2 generated from a peroxidase, H2O2, and halide system. In the HPX-XOD reaction, it was proved that melanin doses not interfere with the enzyme reaction. It is confirmed that one of the mechanisms by which melanin protects UV-induced skin damage is likely scavenging or quenching activity against ROS such as O2.- and 1O2.

Original languageEnglish
Pages (from-to)224-228
Number of pages5
JournalJournal of Clinical Biochemistry and Nutrition
Issue number3
Publication statusPublished - 2010 May


  • Antioxidant
  • Melanin
  • Reactive oxygen species

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry


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