Saturated fatty acids stimulate and insulin suppresses CIDE-A expression in bovine mammary epithelial cells

Tomo Yonezawa, Satoshi Haga, Yosuke Kobayashi, Kazuo Katoh, Yoshiaki Obara

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)


    Cell death-inducing DNA fragmentation factor-alpha-like effector A (CIDE-A) was first identified by its sequence homology with the N-terminal domain of DNA fragmentation factor (DFF). CIDE-A negatively regulates the activity of uncoupling protein 1 (UCP1) in brown adipose tissue. CIDE-A and UCP1 mRNA were detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and lactating bovine mammary glands. Physiological concentrations of saturated fatty acids (stearate and palmitate), but not unsaturated fatty acids (oleate and linoleate) induced up-regulation of CIDE-A mRNA in bMEC. Treatment with insulin (5-10 ng/ml) induced down-regulation of CIDE-A and UCP1. The expression levels of CIDE-A and UCP1 mRNA in bovine mammary glands at various stages of the lactation cycle were determined by quantitative RT-PCR analysis. CIDE-A mRNA expression at peak lactation (2 months after parturition) was significantly higher than at dry off and non-pregnancy but not late lactation. These results suggest that CIDE-A and UCP1 are regulated by insulin and/or fatty acids in mammary epithelial cells and lactating mammary glands, and thereby play an important role in lipid and energy metabolism.

    Original languageEnglish
    Pages (from-to)535-539
    Number of pages5
    JournalBiochemical and biophysical research communications
    Issue number4
    Publication statusPublished - 2009 Jul 10


    • Cell death-inducing DFFA-like effector A
    • Insulin
    • Long-chain fatty acids
    • Mammary epithelial cell
    • Uncoupling protein 1

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology


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