Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells

Punnee Nusuetrong, Thitima Pengsuparp, Duangdeun Meksuriyen, Masaaki Tanitsu, Haruhisa Kikuchi, Michinao Mizugaki, Ken Ichi Shimazu, Yoshiteru Oshima, Norimichi Nakahata, Makoto Yoshida

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.

Original languageEnglish
Pages (from-to)1115-1120
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume31
Issue number6
DOIs
Publication statusPublished - 2008 Jun 1

Keywords

  • Apoptosis
  • Glutathione
  • Lipid peroxide
  • Reactive oxygen species
  • Satratoxin H

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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