Runx1 is involved in the fusion of the primary and the secondary palatal shelves

Kesinee Charoenchaikorn, Tomomasa Yokomizo, David P. Rice, Tadashi Honjo, Kiyomi Matsuzaki, Yuko Shintaku, Yuichi Imai, Asami Wakamatsu, Satoru Takahashi, Yoshiaki Ito, Teruko Takano-Yamamoto, Irma Thesleff, Masayuki Yamamoto, Takashi Yamashiro

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Runx1 is expressed in medial edge epithelial (MEE) cells of the palatal shelf. Conditionally rescued Runx1-/- mice showed limited clefting in the anterior junction between the primary and the secondary palatal shelves, but not in the junction between the secondary palates. In wild type mice, the fusing epithelial surface exhibited a rounded cobblestone-like appearance, while such cellular prominence was less evident in the Runx1 mutants. We also found that Fgf18 was expressed in the mesenchyme underlying the MEE and that locally applied FGF18 induced ectopic Runx1 expression in the epithelium of the palatal explants, indicating that Runx1 was induced by mesenchymal Fgf18 signaling. On the other hand, unpaired palatal explant cultures revealed the presence of anterior-posterior (A-P) differences in the MEE fates and fusion mechanism. Interestingly, the location of anterior clefting in Runx1 mutants corresponded to the region with different MEE behavior. These data showed a novel function of Runx1 in morphological changes in the MEE cells in palatal fusion, which is, at least in part, regulated by the mesenchymal Fgf signaling via an epithelial-mesenchymal interaction.

Original languageEnglish
Pages (from-to)392-402
Number of pages11
JournalDevelopmental Biology
Volume326
Issue number2
DOIs
Publication statusPublished - 2009 Feb 15

Keywords

  • Anterior clefting
  • Cleft palate
  • Epithelial fusion
  • Epithelial-mesenchymal interactions
  • Fgf18
  • Palatal explants
  • Palatogenesis
  • Primary palate
  • Runx1
  • Secondary palate

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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