RPT2/NCH1 subfamily of NPH3-like proteins is essential for the chloroplast accumulation response in land plants

Noriyuki Suetsugu, Atsushi Takemiya, Sam Geun Kong, Takeshi Higa, Aino Komatsu, Ken Ichiro Shimazaki, Takayuki Kohchi, Masamitsu Wada

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

In green plants, the blue light receptor kinase phototropin mediates various photomovements and developmental responses, such as phototropism, chloroplast photorelocation movements (accumulation and avoidance), stomatal opening, and leaf flattening, which facilitate photosynthesis. In Arabidopsis, two phototropins (phot1 and phot2) redundantly mediate these responses. Two phototropininteracting proteins, NONPHOTOTROPIC HYPOCOTYL 3 (NPH3) and ROOT PHOTOTROPISM 2 (RPT2), which belong to the NPH3/RPT2-like (NRL) family of BTB (broad complex, tramtrack, and bric à brac) domain proteins, mediate phototropism and leaf flattening. However, the roles of NRL proteins in chloroplast photorelocation movement remain to be determined. Here, we show that another phototropininteracting NRL protein, NRL PROTEIN FOR CHLOROPLAST MOVEMENT 1 (NCH1), and RPT2 redundantly mediate the chloroplast accumulation response but not the avoidance response. NPH3, RPT2, and NCH1 are not involved in the chloroplast avoidance response or stomatal opening. In the liverwort Marchantia polymorpha, the NCH1 ortholog, MpNCH1, is essential for the chloroplast accumulation response but not the avoidance response, indicating that the regulation of the phototropin-mediated chloroplast accumulation response by RPT2/NCH1 is conserved in land plants. Thus, the NRL protein combination could determine the specificity of diverse phototropinmediated responses.

Original languageEnglish
Pages (from-to)10424-10429
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number37
DOIs
Publication statusPublished - 2016 Sep 13
Externally publishedYes

Keywords

  • Arabidopsis
  • Blue light
  • Chloroplast movement
  • Marchantia
  • Phototropin

ASJC Scopus subject areas

  • General

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