Roles of Pax-2 in initiation of the chick tectal development

Tatsuya Okafuji, Jun Ichi Funahashi, Harukazu Nakamura

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Transplantation experiments have shown that the mes-metencephalic boundary (isthmus) acts as an organizer for the development of the optic tectum. We have cloned Pax-2 which is expressed in the isthmus. Previously it was shown that Pax-5, a member of the same Pax subfamily as Pax-2, transformed the diencephalon into a tectum-like structure and induced isthmus- and tectum-related genes both in the mesencephalon and in the diencephalon. In order to define the distinct roles between Pax-2 and Pax-5 in development of the tectum, we expressed Pax-2 ectopically in the mesencephalon and the diencephalon of E2 chick embryos by in ovo electroporation. Histological observation demonstrated that Pax-2 transformed the diencephalon into a tectum-like structure. In Pax-2, transfected embryos the expression of isthmus- and tectum-related genes such as Fgf8 and En-2 was induced in the diencephalon. However, neither Fgf8 nor En-2 expression was induced in the mesencephalon, making a striking contrast with the result of Pax-5 misexpression. In E2 chick embryos, the mesencephalon is committed of its fate to differentiate into the tectum, but the diencephalon has plasticity on its fate. Moreover, Pax-2 expression in the isthmus precedes Pax-5 expression. Taking these results into consideration, it is suggested that Pax-2 plays a crucial role in initiation of the tectal development, and that Pax-5 functions to maintain the state of tectal differentiation. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalDevelopmental Brain Research
Volume116
Issue number1
DOIs
Publication statusPublished - 1999 Aug 5
Externally publishedYes

Keywords

  • Chick embryo
  • Isthmus
  • Optic tectum
  • Organizer
  • Pax-2
  • Pax-5

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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