Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast

Shiho Makino, Yuichiro Mishima, Kunio Inoue, Toshifumi Inada

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Background: Animal microRNAs silence their target mRNAs by promoting mRNA degradation and inhibiting translation via GW182/TNRC6. Results: TNRC6 induces silencing effects in S. cerevisiae via CCR4-NOT complex and Dhh1-Pat1 when tethered to reporter mRNAs. Conclusion: TNRC6 utilizes the conserved mRNA fate modulators for gene silencing in yeast. Significance: Yeast genetic tools are now available to study intricate actions of TNRC6.

Original languageEnglish
Pages (from-to)8331-8347
Number of pages17
JournalJournal of Biological Chemistry
Volume290
Issue number13
DOIs
Publication statusPublished - 2015 Mar 27

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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