Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells

Yohei Oishi, Toshiya Arakawa, Akihiko Tanimura, Makoto Itakura, Masami Takahashi, Yoshifumi Tajima, Itaru Mizoguchi, Taishin Takuma

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We evaluated the role of VAMP-2/synaptobrevin, VAMP-7/TI-VAMP, and VAMP-8/endobrevin in exocytic pathways of HSY cells, a human parotid epithelial cell line, by coexpressing these VAMP proteins tagged with green fluorescent protein (GFP) and human growth hormone (hGH) as a secretory cargo. Exocytosis of hGH was constitutive and the fluorescent signal of hGH-GFP was observed in the Golgi area and small vesicles quickly moving throughout the cytoplasm. The cytoplasmic vesicles containing hGH overlapped well with VAMP-7-GFP, but did so scarcely with VAMP-2-GFP or VAMP-8-GFP. However, when the vesicle transport from the trans-Golgi network to the plasma membrane was arrested by incubation at 20°C for 2 h and then released by warming up to 37°C; VAMP-2-GFP and hGH were clearly colocalized together in small cytoplasmic vesicles. Neither VAMP-7-GFP nor hGH-GFP was colocalized with LAMP-1, a marker for lysosomes and late endosomes. These results suggest that (1) VAMP-2 can be one of the v-SNAREs for constitutive exocytosis; (2) VAMP-7 is involved in the constitutive exocytosis as a slow, minor v-SNARE, but not in the lysosomal transport; and (3) VAMP-8 is unlikely to be a v-SNARE for constitutive exocytosis in HSY cells.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalHistochemistry and Cell Biology
Volume125
Issue number3
DOIs
Publication statusPublished - 2006 Mar
Externally publishedYes

Keywords

  • Constitutive exocytosis
  • SNARE
  • VAMP-2
  • VAMP-7
  • VAMP-8
  • hGH-GFP

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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