Role of vacuolar H+-inorganic pyrophosphatase in tomato fruit development

Seedahmed A. Mohammed, Sogo Nishio, Hideyuki Takahashi, Katsuhiro Shiratake, Hiroki Ikeda, Koki Kanahama, Yoshinori Kanayama

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    cDNA corresponding to two type-I vacuolar H+-inorganic pyrophosphatases (V-PPases) (SlVP1, SlVP2) and one type-II V-PPase (SlVP3) was isolated from tomato fruit to investigate their role in fruit development. Southern analysis revealed that type-I V-PPase genes form a multigene family, whereas there is only one type-II V-PPase gene in the tomato genome. Although SlVP1 and SlVP2 were differentially expressed in leaves and mature fruit, the highest levels of both SlVP1 and SlVP2 mRNA were observed in fruit at 24 days after anthesis. The expression pattern of type-II SlVP3 was similar to that of SlVP2, and the highest levels of SlVP3 mRNA were also observed in fruit at 24 days after anthesis, thus suggesting that SlVP3 plays a role in early fruit development. Because SlVP1 and SlVP2 mRNA was more abundant than SlVP3 mRNA, expression of type-I V-PPases was analysed further. Type-I V-PPase mRNA was localized in ovules and their vicinities and in vascular tissue at an early stage of fruit development. Tomato RNAi lines in which the expression of type-I V-PPase genes was repressed using the fruit-specific promoter TPRP-F1 exhibited fruit growth retardation at an early stage of development. Although the major function of V-PPases in fruit has been believed to be the accumulation of materials such as sugars and organic acids in the vacuole during cell expansion and ripening, these results show that specific localization of V-PPase mRNA induced by pollination has a novel role in the cell division stage.

    Original languageEnglish
    Pages (from-to)5613-5621
    Number of pages9
    JournalJournal of experimental botany
    Volume63
    Issue number15
    DOIs
    Publication statusPublished - 2012 Sep

    Keywords

    • Fruit development
    • V-PPase
    • tomato
    • vacuolar H+-inorganic pyrophosphatase

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

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