Role of the G-box element in regulation of chlorophyll biosynthesis in arabidopsis roots

Koichi Kobayashi, Takeshi Obayashi, Tatsuru Masuda

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Regulation of chlorophyll metabolism comprises strong transcriptional control together with a range of post-translational mechanisms during chloroplast biogenesis. Recently we reported that chlorophyll biosynthesis in Arabidopsis thaliana roots is regulated by auxin/cytokinin signaling via the combination of two transcription factors, LONG-HYPOCOTYL5 (HY5) and GOLDEN2-LIKE2 (GLK2). In this study, we examined the involvement of cis-elements in the expression of chlorophyll biosynthesis genes. Searches for predicted cis-elements in key chlorophyll biosynthesis genes and their coexpressed genes revealed coexistence of the G-box motif and the CCAATC motif, which may be targeted by HY5 and GLK factors, respectively, in their promoter regions. Deletion of the G-box from the promoter of the CHLH gene encoding the H subunit of Mg-chelatase resulted in the absence of its expression in roots but not in shoots, showing a differing involvement of the G-box in CHLH expression between shoots and roots. Our data suggest that transcription factors and cis-elements participating chlorophyll biosynthesis are substantially changed during organ differentiation, which may be linked to the differentiation of plastids.

Original languageEnglish
JournalPlant Signaling and Behavior
Volume7
Issue number8
DOIs
Publication statusPublished - 2012 Aug

Keywords

  • Chlorophyll biosynthesis
  • Cis-element
  • Co-expression network
  • Photosynthesis
  • Transcriptional regulation

ASJC Scopus subject areas

  • Plant Science

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