Role of the carboxy-terminal region of the outer membrane protein AatA in the export of dispersin from enteroaggregative Escherichia coli

Mayumi Iwashita, Junichiro Nishi, Naoko Wakimoto, Rika Fujiyama, Kimie Yamamoto, Koichi Tokuda, Kunihiro Manago, Yoshifumi Kawano

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen in both developing and industrialized countries. AatA, an outer-membrane protein that is a homolog of E. coli TolC, facilitates the export of the dispersin protein Aap across the outer membrane in EAEC. To identify which amino acids are important for this export activity, site-directed mutagenesis of the carboxy terminus was performed. An insertional mutant of aatA was complemented with each of several deletion mutants, and was examined for Aap secretion. The results showed that three nonpolar amino acids at positions 381-383 (Phe-Leu-Leu) were required for the activity, and these residues were located at the base of carboxy-terminal elongation in the equatorial domain of AatA.

Original languageEnglish
Pages (from-to)266-272
Number of pages7
JournalFEMS Microbiology Letters
Volume256
Issue number2
DOIs
Publication statusPublished - 2006 Mar 1
Externally publishedYes

Keywords

  • AatA
  • Dispersin
  • Enteroaggregative Escherichia coli (EAEC)
  • Site-directed mutagenesis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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