Role of the 33-kDa polypeptide in preserving Mn in the photosynthetic oxygen-evolution system and its replacement by chloride ions

Mitsue Miyao, Norio Murata

Research output: Contribution to journalArticlepeer-review

240 Citations (Scopus)

Abstract

Treatment of Photosystem II particles from spinach chloroplasts with Triton X-100 with 2.6 M urea in the presence of 200 mM NaCl removed 3 polypeptides of 33 kDa, 24 kDa and 18 kDa, but left Mn bound to the particles. The (urea + NaCl)-treated particles could evolve oxygen in 200 mM, but not in 10 mM NaCl. Mn was gradually released with concomitant loss of oxygen-evolution activity in 10 mM NaCl but not in 200 mM Cl-. The NaCl-treated particles, which contained Mn and the 33-kDa polypeptide but not the 24-kDa and 18-kDa polypeptides, did not lose Mn or oxygen-evolution activity in 10 mM NaCl. These observations suggest that the 33-kDa polypeptide maintains the binding of Mn to the oxygen-evolution system and can be functionally replaced by 200 mM Cl-.

Original languageEnglish
Pages (from-to)350-354
Number of pages5
JournalFEBS Letters
Volume170
Issue number2
DOIs
Publication statusPublished - 1984 May 21
Externally publishedYes

Keywords

  • 33-kDa polypeptide
  • Cl
  • Mn
  • Oxygen evolution
  • Photosynthesis
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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